Coding

Part:BBa_K5226006:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-07-28)


TD-gcvP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1564
    Illegal PstI site found at 1841
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1662
    Illegal SpeI site found at 1564
    Illegal PstI site found at 1841
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1564
    Illegal PstI site found at 1841
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1564
    Illegal PstI site found at 1841
    Illegal NgoMIV site found at 291
    Illegal NgoMIV site found at 1636
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

Sequence source is from Halomonas sp. TD01 strain, found through NCBI （https://www.ncbi.nlm.nih.gov/nuccore/OV350343.1?from=3295860&to=3298754）

References